Genetic characterization of grapevine-infecting Botrytis cinerea isolates from Argentina.

CLAUDIO MUÑOZ, SEBASTIÁN GÓMEZ TALQUENCA, ENRIQUE ORIOLANI AND MARIANA COMBINA

REVISTA IBEROAMERICANA DE MICOLOGIA

Elsevier

Año: 2010 vol. 27 p. 66 - 70

1130-1406

SummaryBackground: Botrytis cinerea is an ascomycete with a high genetic diversity and complex population structure, as reported from several hosts and places, but nothing is know about its genetic diversity in Argentina.Aims: The aim of this work is to estimate the genetic diversity of a local population of B.cinerea isolates obtained from grapevine in Argentina.Methods: In this work, 35 strains that have isolated from grapevines, were genotyped for the presence of transposable elements and PCR-based RFLP molecular markers. The obtained results were used to perform a population genetics analysis using the Genepop software.Results: All isolates analyzed were classified as Group II (according to the most recent proposed classification) and showed a high degree of genetic diversity, with 15 different haplotypes. The data obtained were compared with those for a large French population of the fungus, and a notable difference in allele frequency was recorded.Conclusions: These differences between the compared populations, led to a high level of population diversity and differentiation between the local and French groups of isolates. This was confirmed by an Fst value of 0.3332, which was higher than reported previously for other pair wise comparisons of populations. This work represent the first to report to the genetic diversity of B.cinerea isolates and their population structure in Argentina.