IBAM 22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
artículos
Título:
Phenolic metabolites in plasma and tissues of rats fed with a grape pomace extract as assessed by liquid chromatography-tandem mass spectrometry
Autor/es:
PERDICARO, DIAHANN J.; VAZQUEZ PRIETO, MARCELA A.; PERDICARO, DIAHANN J.; VAZQUEZ PRIETO, MARCELA A.; RODRIGUEZ LANZI, CECILIA; ANTONIOLLI, ANDREA; PICCOLI, PATRICIA; FONTANA, ARIEL; RODRIGUEZ LANZI, CECILIA; ANTONIOLLI, ANDREA; PICCOLI, PATRICIA; FONTANA, ARIEL
Revista:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2018 vol. 651 p. 28 - 33
ISSN:
0003-9861
Resumen:
Grape pomace extract (GPE) is a rich and relatively low-cost source of phenoliccompounds. However, little is known about the main GPE metabolites in mammals,which could help explain the observed health-promoting effects. This studyinvestigated the presence of parent compounds from flavanol, flavonol and stilbene families and their metabolites in rat plasma and tissues after an acute intake of GPE in doses of 300 and 600 mg kg/body weight. The measurement of freecompounds and their metabolites was performed by ultra-high performance liquidchromatography-tandem mass spectrometry (UHPLC-MS/MS). Results showedthe presence of epicatechin, epicatechin methyl-glucuronide, epicatechin methylsulphate, catechin, catechin-glucuronide, quercetin methyl-glucuronide,resveratrol-3-glucuronide, resveratrol-4-glucuronide and resveratrol-3-sulphate in plasma, which was dose dependent. The most abundant measured compound inplasma was epicatechin-glucuronide. The presence of glucuronidated and methylglucuronidated forms of catechin were observed in the liver at both doses, while epicatechin-glucuronide and methyl-glucuronide were detected only upon intake of 600 mg GPE/kg body weight. At this dose epicatechin-glucuronide and methylglucuronide were also detected in muscle, and catechin methyl-glucuronide in adipose tissue. Results show the main GPE metabolites present in rat tissues after oral consumption, contributing to better understand the health benefits of GPE and its potential utilization as a functional ingredient.
