IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
artículos
Título:
QuEChERS method for the determination of 3‑alkyl‑2‑methoxypyrazines in wines by gas chromatography-mass spectrometry
Autor/es:
BOTTINI, R.; FONTANA, A.
Revista:
FOOD ANALYTICAL METHODS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2016 vol. 9 p. 3352 - 3359
ISSN:
1936-9751
Resumen:
A new method for determining 3-isopropyl-2-methoxypyrazine (IPMP), 3-sec-butyl-2-methoxypyrazine (SBMP) and 3-isobutyl-2-methoxypyrazine (IBMP) in wines is presented. A modified quick, easy, cheap, effective, rugged and safe (QuEChERS) method and gas chromatography-mass spectrometry (GC-MS) were used for sample preparation and compounds determination, respectively. The analytes were extracted from wine (30 mL) with 1 mL toluene, in presence of 12 g anhydrous MgSO4 and 3 g NaCl. Clean-up of the toluene phase was performed by a miniaturized dispersive solid-phase extraction (d-SPE) with a combination of anhydrous CaCl2 (25 mg), anhydrous MgSO4 (25 mg) and primary-secondary amine (PSA, 10 mg), which was effective for minimizing co-extractives and matrix effects. GC-MS parameters were also tuned-up to optimize limits of detection (LODs) between 4.2 and 7.1 ng L-1. The overall recoveries (trueness) of the method ranged between 71 and 87% for white and red wine samples respectively, spiked at 40 and 100 ng L-1, with relative standard deviations (RSDs) below 21%. The method was applied for the determination of target MPs in samples of commercial wines from Argentina.