High-throughput modified QuEChERS method for the determination of the mycotoxin tenuazonic acid in wine grapes

MORATA, VILMA; PRENDES, LUCIANA; PRENDES, LUCIANA; FONTANA, A.; BOTTINI, R.; FONTANA, A.; BOTTINI, R.; MORATA, VILMA

RSC Advances

The Royal Society of Chemistry

Lugar: Cambridge; Año: 2016 vol. 6 p. 95670 - 95679

A reliable, simple, fast, inexpensive and robust sample preparation approach for tenuazonic acid (TA) determination in grapes by liquid chromatography with ultraviolet detection (HPLC-UV) is proposed. The method is based on a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach using ethyl acetate as extraction solvent. Its involves extraction of 2.5 g sample (plus 2.5 mL 1% formic acid acidified water) with 5 mL of ethyl acetate; drying/cleaning-up of extract with 0.25 g anhydrous CaCl2 and determination by HPLC-UV. The method was optimized and validated achieving a limit of quantification (LOQ) of 0.05 µg g-1. The overall recoveries were 96 %, 82 % and 97 % for grape samples spiked at 0.05, 0.5 and 5 µg g-1, respectively. The method showed excellent reproducibility with RSDs for the above data ≤ 8 % and Hortwitz ratios