IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
artículos
Título:
Molecular Relationships Between Saccharomyces cerevisiae
Autor/es:
MERCADO, L.; JUBANY, S; GAGGERO, K; MASUELLI, R.W.; COMBINA, M.
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2010 p. 510 - 514
ISSN:
0343-8651
Resumen:
Three molecular typing techniques were
applied to assess the molecular relationships of Saccharomyces
cerevisiae strains isolated from winery equipment,
grapes, and spontaneous fermentation in a cellar located in
Zona Alta del Rý´o Mendoza (Argentina). In addition,
commercial Saccharomyces strains widely used in this
region were also included. Interdelta PCR typing, mtDNA
restriction analysis, and microsatellite (SSR) genotyping
were applied. Dendrograms were constructed based on
similarity among different patterns of bands. The combination
of the three techniques discriminated 34 strains
among the 35 isolates. The results of this study show the
complex relationships found at molecular level among the
isolates that share the same ecological environment, i.e.,
the winemaking process. With a few exceptions, the yeast
isolates were generally clustered in different ways,
depending on the typing technique employed. Three clusters
were conserved independently of the molecular
method applied. These groups of yeasts always clustered
together and had high degree of similarity. Furthermore,
the dendrograms mostly showed clusters combining strains
from winery and fermentation simultaneously. Most of the
commercial strains included in this study were clustered
separately from the other isolates analyzed, and just a few
of them grouped with the strains mainly isolated from
spontaneous fermentation. Only one commercial strain was
clustered repetitively with a noncommercial strain isolated
from spontaneous fermentation in the three dendrograms.
On the other hand, this study has demonstrated the
importance of selecting an appropriate molecular method
according to the main objectives of the research.Saccharomyces
cerevisiae strains isolated from winery equipment,
grapes, and spontaneous fermentation in a cellar located in
Zona Alta del Rý´o Mendoza (Argentina). In addition,
commercial Saccharomyces strains widely used in this
region were also included. Interdelta PCR typing, mtDNA
restriction analysis, and microsatellite (SSR) genotyping
were applied. Dendrograms were constructed based on
similarity among different patterns of bands. The combination
of the three techniques discriminated 34 strains
among the 35 isolates. The results of this study show the
complex relationships found at molecular level among the
isolates that share the same ecological environment, i.e.,
the winemaking process. With a few exceptions, the yeast
isolates were generally clustered in different ways,
depending on the typing technique employed. Three clusters
were conserved independently of the molecular
method applied. These groups of yeasts always clustered
together and had high degree of similarity. Furthermore,
the dendrograms mostly showed clusters combining strains
from winery and fermentation simultaneously. Most of the
commercial strains included in this study were clustered
separately from the other isolates analyzed, and just a few
of them grouped with the strains mainly isolated from
spontaneous fermentation. Only one commercial strain was
clustered repetitively with a noncommercial strain isolated
from spontaneous fermentation in the three dendrograms.
On the other hand, this study has demonstrated the
importance of selecting an appropriate molecular method
according to the main objectives of the research.strains isolated from winery equipment,
grapes, and spontaneous fermentation in a cellar located in
Zona Alta del Rý´o Mendoza (Argentina). In addition,
commercial Saccharomyces strains widely used in this
region were also included. Interdelta PCR typing, mtDNA
restriction analysis, and microsatellite (SSR) genotyping
were applied. Dendrograms were constructed based on
similarity among different patterns of bands. The combination
of the three techniques discriminated 34 strains
among the 35 isolates. The results of this study show the
complex relationships found at molecular level among the
isolates that share the same ecological environment, i.e.,
the winemaking process. With a few exceptions, the yeast
isolates were generally clustered in different ways,
depending on the typing technique employed. Three clusters
were conserved independently of the molecular
method applied. These groups of yeasts always clustered
together and had high degree of similarity. Furthermore,
the dendrograms mostly showed clusters combining strains
from winery and fermentation simultaneously. Most of the
commercial strains included in this study were clustered
separately from the other isolates analyzed, and just a few
of them grouped with the strains mainly isolated from
spontaneous fermentation. Only one commercial strain was
clustered repetitively with a noncommercial strain isolated
from spontaneous fermentation in the three dendrograms.
On the other hand, this study has demonstrated the
importance of selecting an appropriate molecular method
according to the main objectives of the research.Saccharomyces strains widely used in this
region were also included. Interdelta PCR typing, mtDNA
restriction analysis, and microsatellite (SSR) genotyping
were applied. Dendrograms were constructed based on
similarity among different patterns of bands. The combination
of the three techniques discriminated 34 strains
among the 35 isolates. The results of this study show the
complex relationships found at molecular level among the
isolates that share the same ecological environment, i.e.,
the winemaking process. With a few exceptions, the yeast
isolates were generally clustered in different ways,
depending on the typing technique employed. Three clusters
were conserved independently of the molecular
method applied. These groups of yeasts always clustered
together and had high degree of similarity. Furthermore,
the dendrograms mostly showed clusters combining strains
from winery and fermentation simultaneously. Most of the
commercial strains included in this study were clustered
separately from the other isolates analyzed, and just a few
of them grouped with the strains mainly isolated from
spontaneous fermentation. Only one commercial strain was
clustered repetitively with a noncommercial strain isolated
from spontaneous fermentation in the three dendrograms.
On the other hand, this study has demonstrated the
importance of selecting an appropriate molecular method
according to the main objectives of the research.