Solasodine production in transformed organ cultures (roots and shoots) of Solanum eleagnifolium Cav.

ALVAREZ, M.A.; TALOU, J.RODRIGUEZ; PANIEGO, N.B.; GIULIETTI, A.M.

BIOTECHNOLOGY LETTERS

SPRINGER

Año: 1994 vol. 16 p. 393 - 396

0141-5492

Transformed organ cultures of Solanum eleagnifolium Cav. (roots and shoots) were obtained by infection with different Agrobacterium species (A. rhizogenes and A. tumefaciens). The growth index and the solasodine productivity of both kinds of cultures were determinated for several clones. Transformed roots (clone 5) shows a Growth Index of 39.07 and a solasodine yield of 1.90 ± 0.08 mg g-1DW . Solasodine content in clone 5 of transformed roots (1.9 ± 0.08 mg g-1 DW) is similar to those obtained by hormonal manipulation (1.00 - 1.68 mg g-1DW) but higher than those found in shoots and leaves of the wild-grown plant (0.3-0.4 mg g-1DW). © 1994 Chapman & Hall.