Biosynthesis of 2,3-dihydroxybenzoic acid in transgenic Catharathus roseus cell cultures overexpressing isochorismate synthase.

MULJONO, RAB,; RODRIGUEZ TALOU, J.; SCHEFFER, JJC,; VERPOORTE, R.

HETEROCYCLES

Elsevier

Año: 2002 vol. 56 p. 341 - 352

0385-5414

The involvement of isochorismate synthase (EC 5.4.99.6) in the biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. The involvement of isochorismate synthase (EC 5.4.99.6) in the biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. 13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate.