INVESTIGADORES
RODRIGUEZ TALOU Julian
congresos y reuniones científicas
Título:
Phytofermentation processes in stirred tank bioreactors by Rubia tinctorum suspension cultures.
Autor/es:
BUSTO VD; CALABRO LOPEZ A; RODRÍGUEZ TALOU J; GIULIETTI AM; MERCHUK J
Lugar:
Potrero de Funes, San Luis
Reunión:
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Considering the
complexity in the
chemical synthesis of
some secondary products,
plant cell cultures represent an alternative for industrial application.
However, an impediment of the
practice of large-scale
culture in stirred
tanks is the
shear stress. Rubia
tinctorum suspension
cultures produce anthraquinones (AQs)
which are important
in the pharmaceutical and food
industries. The aim of this work was to study the performance of R. tinctorum
suspension cultures in a stirred
tank bioreactor including
the evaluation of shear
stress effects. A 1.5 L
stirred tank bioreactor
agitated at 150,
450 and 800
rpm was used. Controls
were carried out
in 100 mL
shake flasks agitated
at 100 rpm. Cell
growth and AQs production
were evaluated. Specific
growth rate (u)
attained in shake
flasks cultures was 27% and 42% higher than those obtained in the
bioreactor cultures at 450 and 150 rpm, respectively. Cells cultured at 800 rpm
were not able to grow into the bioreactor. Cell
cultures were mechanically
elicitated by shear
stress at 450
and 800 rpm
increasing AQs biosynthesis by
230% and 243%,
respectively. AQs volumetric
productivity (Qp) at 450
was 167% higher
than that achieved
in shake flasks.
R. tinctorum cells
showed moderately sensitivity in
phytofermentative processes. The metabolic capabilities showed by these
plant cells could be used as a potential strategy for industrial AQs production