Anisodamine production by Argentinean and Colombian Brugmansia candida in vitro cultures

CARDILLO AB; OTALVARO MA; CALABRO A; VELAZQUEZ ME; RODRIGUEZ TALOU J.; GIULIETTI AM

Rosario

Simposio; VII Simposio Nacional de Biotecnología REDBIO-Argentina; II Congreso Internacional-REDBIO-Argentina; 2009

REDBIO 2009

Study the anisodamine (2) content in Argentinean and Colombian B. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. B. candida in vitro germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one. germinated seedlings as well as the production of this alkaloid by hairy root cultures obtained from them. Also, the hairy root tropane alkaloid profile (hyoscyamine, anisodamine and scopolamine, 1 - 3) was compared to the plant one.