RODRIGUEZ TALOU Julian
congresos y reuniones científicas
Anthraquinones elicitation and oxidative burst in cell suspensions Rubia tinctorum.
PERASSOLO M; BUSTO VD; QUEVEDO CV; CARDILLO, AB; MARTÍNEZ CA; CUADRADO VL; MERINI LJ; RODRÍGUEZ TALOU J
Mar del Plata, Argentina
Congreso; XLIII Congreso de SAIB, Noviembre 2007, .; 2007
In cell suspensions, anthraquinones (AQs) production is induced by elicitors such as chitosan. This defense response also includes generation of reactive oxygen species (ROS) and cell death. It has been recently showed that chitosan induction of anthraquinone synthesis in involves the stimulation of PLC, intracellular Ca mobilization and PI3K, which mediatesMAPKactivation. In the present work we study the relationship between elicitation, oxidative burst and cell death. Elicitation with chitosan (200 mg/l) stimulates AQs synthesis in L. cultures at 24 and 48 hr. An increase in H O production at 6 and 24 hr was observed and correlated with higher levels of cell death.Addition of chitosan and diphenylene iodonium (DPI), an inhibitor of H O generation, resulted in a decrease in cell death andH O production. In both treatments similar levels ofAQs accumulation were observed. In cultures treated with chitosan and lanthanum chloride, an intracellular calcium channel blocker, cell death and AQs production decreased showing that both processes require an increase in cytosolic calcium levels. The relationship between elicitation andROSgeneration is under study.