RODRIGUEZ TALOU Julian
congresos y reuniones científicas
A. tumefaciens and biolistic mediated transformation of Morinda citrifollia cell suspensions.
QUEVEDO CV; GIULIETTIAM; RODRÍGUEZ TALOU J.
Mar del Plata, Argentina.
Congreso; XLIII Congreso de SAIB, Noviembre 2007,; 2007
Morinda citrifollia accumulate anthraquinones (AQs) which are secondary metabolites with therapeutic potential. AQs are formed from the methylerythritol 4-phosphate (MEP) pathway. 1-deoxy-D-Xylulose-5-phosphate synthase (dxs) the first enzyme in the MEP pathway catalyzes the transketolase reaction converting pyruvate and glyceraldehyde 3-phosphate into 1-deoxy-D-Xylulose-5-phosphate. Also it has been shown that Octadecanoid-Derivative Responsive Catharanthus Apetala 2-domain Protein transcription factors (ORCA) induce the synthesis of specific enzymes from secondary metabolism as well as another kind of response defenses against pathogens. In the present work we used to different methods to obtain transgenic cell lines of M. citrifolia: Agrobacterium mediated transformation and biolistic. Two binary vectors were used, in the first one the cDNA of dxs obtained from Catharanthus roseus, was cloned between the Cauliflower mosaic virus 35S-promoter (CaMV-35S) and the potato proteinase inhibitor terminator (Pit). The cassette expression was inserted into the binary vector pMOG22-GUS, containing the GUS reporter gene. In the second one, the ORCA gene was cloned into the pCAMBIA 1300binary vector containing GFP as a reporter gene. M. citrifollia cell suspensions were transformed by A. Tumefaciens and biolistic. Transformations were checked by expression of the reporter genes, GUS and GFP.