RODRIGUEZ TALOU Julian
congresos y reuniones científicas
Glutamate effects on secondary metabolic pathways in Rubia tinctorum cell suspensions
PERASSOLO M; GIULIETT, A M. AND RODRIGUEZ TALOU, J.
Congreso; XLII Congreso de SAIB; 2006
Sociedad Argentina de Investigaciones Bioquimicas
Shikimate pathway supplies carbon structures for many secondary metabolic pathways in plants. Chorismate, which is the end product of the shikimate pathway, becomes the branch point for the synthesis of phenylpropanoid and anthraquinones (AQs) in Rubia tinctorum secondary metabolism. Proline biosynthesis in plants stimulates the pentose phosphate pathway (PPP) which results in an increasing carbon flux through the shikimate pathway. Proline is synthesized from glutamate by a series of reduction reactions. These enzymatic steps generate NADP+, which is the cofactor required in the PPP. We tested the effect of glutamate addition on plant suspension cultures of R. tinctorum in order to study its effect on secondary metabolic pathways. Suspension cultures treated with glutamate 5 mM showed higher levels of AQs at 4 (30%) and 8 (24%) days of culture. Total phenol contents also increased at 2 (11%) and 4 (11%) days of culture. The stimulation of these metabolic pathways could be correlated with the stimulation of the pentose phosphate pathway.