RODRIGUEZ TALOU Julian
congresos y reuniones científicas
DXS expression in Morinda Citrifolia cell suspension cultures for increasing Antrhaquinones production.
QUEVEDO CARLA, GIULIETTI ANA M AND RODRIGUEZ TALOU JULIÁN.
Congreso; XLII Congreso de SAIB; 2006
Sociedad Argentina de Investigaciones Bioquimicas
The isoprenoid moiety constituting of anthraquinones (AQs) in the plant family Rubiaceae is formed from the methylerythritol 4-phosphate (MEP) pathway. 1-deoxy-D-Xylulose-5-phosphate synthase (DXS), the first enzyme in the (MEP) pathway catalyzes the transketolase reaction converting pyruvate and glyceraldehyde 3-phosphate into 1-deoxy-D-Xylulose-5-phosphate. In this study we constructed an expression vector with DXS to investigate its overexpression in Morinda Citrofolia cell suspension cultures. The dxs cDNA obtained from Catharantus Roseus cloned in a pGemTeasy vector, was digested and ligated between the Cauliflower mosaic virus 35S-promoter (CaMV-35S) and the potato proteinase inhibitor terminator (PIt) of pMOG843 (MOGEN, Leiden, The Netherlands). Subsequently, the cassette expression containing dxs was inserted into the binary vector pMOG22-GUS, which contains the hygromycin resistance gene, the GUS reporter gene and the left and right T-DNA borders from Agrobacterium tumefaciens. M. citrifolia cell cultures were transformed using A. tumefaciens strain LBA4404 containing the compatible plasmid pBRIMCS carrying a constitutive virG gene. Transformation was confirmed by analysis of the GUS reporter gene. Overexpression of dxs gene in transformed cell lines is under study.