RODRIGUEZ TALOU Julian
capítulos de libros
Biosynthesis of 2,3-dihydroxybenzoic acid in transgenic Catharanthus roseus cell cultures overexpressing isochorismate synthase
BUDI MULJONO, RA; RODRIGUEZ TALOU , J; SCHEFFER, JJC,; VERPOORTE, R.
The isochorismate pathway as a route to 2,3-dihydroxybenzoic acid Catharanthus roseus cell cultures
Lugar: Leiden; Año: 2001; p. 81 - 94
Abstract [I -'3~]pyruvate feeding has been used to examine the biosynthetic pathway of 2,3 -dihydroxybenzoic acid (2,3-DHBA) in Catharanthus roseus cell cultures . Preliminary experiments with feeding of labeled p yruvate or phenylalanine to an elicited wild type C. roseus cell culture did not result in incorporation of any label in 2,3-DHBA. To test the involvement of isochorismate synthase in the biosynthesis of 2,3-DHBA, we repeated the experiment with a transgenic C. roseus cell line which constitutively overexpressed the C. roseus plant ics-gene (ICS = isochorismate synthase, E.C. 22.214.171.124). Higher levels of 2,3-DHBA were detected after elicitation in the transgenic cell lines if compared with untransformed C. roseus cell line. Moreover, this cell line did show incorporation of labeled [ 1- '3~]pyruvate.The label was mainly found in the position C-7 (carboxyl group) of 2,3-DHBA, but also C-3 and C-4 did show some incorporation. This pattern is consistent with isochorismate as intermediate in the bios ynthesis of 2,3-DHB A and confirms the involvement of ICS in this pathway.