INVESTIGADORES
RODRIGUEZ TALOU Julian
artículos
Título:
In vitro cytotoxic and antioxidant activity of extracts of Baccharis coridifolia D.C
Autor/es:
MONGELLI , E; DESMARCHELIER , C; RODRIGUEZ TALOU , J; COUSSIO, J; CICCIA, G
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
Elsevier
Referencias:
Año: 1997 vol. 48 p. 157 - 157
ISSN:
0378-8741
Resumen:
The in vitro antioxidant and cytotoxic activities in different extracts of Baccharis coridifolia DC. (Compositae) were studied using different bioassays. Antioxidant activity was tested using hydroperoxide-initiated chemiluminescence (CL) in rat liver homogenates. The aqueous extract showed antioxidant activity (IC50= 141 pg/ml), while the dichloromethane and methanol extracts were prooxidant. Activity in the aqueous extract was confirmed by determination of thiobarbituric acid-reactive substances (TBARS), showing an IC5o = 556/~g/ml. On the other hand, studies on the cytotoxic activity demonstrated a 78% crown gall tumour inhibition and an ED50 = 4. 2 pg/ml in KB cells growth for the dichloromethane extract. A 56% decrease in the initial absorbance of DNA-Methyl Green complex could also be observed at 1000 /~g/ml, suggesting the presence of compounds that interact with DNA. © 1997 Elsevier Science Ireland Ltd. studied using different bioassays. Antioxidant activity was tested using hydroperoxide-initiated chemiluminescence (CL) in rat liver homogenates. The aqueous extract showed antioxidant activity (IC50= 141 pg/ml), while the dichloromethane and methanol extracts were prooxidant. Activity in the aqueous extract was confirmed by determination of thiobarbituric acid-reactive substances (TBARS), showing an IC5o = 556/~g/ml. On the other hand, studies on the cytotoxic activity demonstrated a 78% crown gall tumour inhibition and an ED50 = 4. 2 pg/ml in KB cells growth for the dichloromethane extract. A 56% decrease in the initial absorbance of DNA-Methyl Green complex could also be observed at 1000 /~g/ml, suggesting the presence of compounds that interact with DNA. © 1997 Elsevier Science Ireland Ltd. Baccharis coridifolia DC. (Compositae) were studied using different bioassays. Antioxidant activity was tested using hydroperoxide-initiated chemiluminescence (CL) in rat liver homogenates. The aqueous extract showed antioxidant activity (IC50= 141 pg/ml), while the dichloromethane and methanol extracts were prooxidant. Activity in the aqueous extract was confirmed by determination of thiobarbituric acid-reactive substances (TBARS), showing an IC5o = 556/~g/ml. On the other hand, studies on the cytotoxic activity demonstrated a 78% crown gall tumour inhibition and an ED50 = 4. 2 pg/ml in KB cells growth for the dichloromethane extract. A 56% decrease in the initial absorbance of DNA-Methyl Green complex could also be observed at 1000 /~g/ml, suggesting the presence of compounds that interact with DNA. © 1997 Elsevier Science Ireland Ltd.