RODRIGUEZ TALOU Julian
Synergistic effect of methyl jasmonate and cyclodextrins on anthraquinone accumulation in cell suspension cultures of Morinda citrifolia and Rubia tinctorum
PERASSOLO M; SMITH E; GIULIETTI AM; RODRÍGUEZ TALOU J
PLANT CELL TISSUE AND ORGAN CULTURE
Lugar: Berlin; Año: 2015
Plant in vitro culture is a platform for produc-ing secondary metabolites that combines safety, quality and low environmental impact. Besides, it is possible to increase the accumulation of these compounds by different strategies, such as elicitation. In this work, we analyzed the effects of the combination of methyl jasmonate (MeJ) and two cyclodextrins (CDs) on the production of anthraqui-nones (AQs) in cell cultures of Rubiaceae (Morinda citrifolia and Rubia tinctorum). These secondary metabolites have been traditionally used as dyes and have interesting therapeutic applications. The experiments were designed according to a full factorial design of two factors (MeJ and a CD) in two levels (0 and 0.1 mM for MeJ, and 0 and 20 mM of the CD). MeJ and (2-hydroxypropyl)-b-cy-clodextrin (HPCD) synergistically increased intracelular AQ content in suspension cultures ofR. tinctorum, and, to a lesser extent, in suspension cultures of M. citrifolia. Combination of MeJ with another CD, 2-methyl-b-cy-clodextrin, led to a more intense and later increase in AQ content in cell cultures ofR. tinctorumwhen compared to MeJ?HPCD treatment. However, the combination of CD and MeJ failed to induce a drastic AQ release to the cultura media. Nevertheless, our results show that combination of strategies (using a CD and MeJ) was successful to increase secondary metabolite accumulation in suspension cultures. To our knowledge, this is the first report of synergistic effect of MeJ and CD on AQ accumulation in plant in vitro cultures.