RODRIGUEZ TALOU Julian
Exploring different strategies to express Dengue virus envelope protein in a plant system
MARTÍNEZ, CA; TOPAL, E; GIULIETTI, AM; RODRIGUEZ TALOU , J; MASON, H
Año: 2010 vol. 32 p. 867 - 867
Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.