RAPISARDA Viviana Andrea
congresos y reuniones científicas
UV-B LIGHT ENHANCES ANTIMICROBIAL ACTIVITY OF POSTHARVEST LEMON PEEL AGAINST PENICILLIUM DIGITATUM
RUIZ, V.E.; CERIONI, L.; ZAMPINI, C.; BOUZO, C.; ISLA, M. I.; HILAL, M.; RAPISARDA, V. A.
Congreso; LII Annual Meeting of SAIB (Argentine Society for Biochemistry and Molecular Biology); 2016
UV-B radiation treatment previously standardized in our laboratory contributes to prevent green mold on postharvest lemons, caused by Penicillium digitatum. The aim of this work was to assess in vitro the effect of flavedo extracts from UV-B exposed lemons (UV-B FEx) and from control lemons (CFEx) onP. digitatum conidia. Several parameters of conidia viability were evaluated after treatments. Moreover, HPLC and HPLC MS/MS analysis of phenolic compounds were carried out on both flavedo extracts. Conidia viability was markedly affected by UV-B FEx in respect to CFEx, in assays using the same concentrations. Conidia treated with UV-B FEx showed an inhibition of germination and oxygen consumption, an increased membrane permeability and ROS and TBARS production. On the other hand, two fractions of the flavedo extracts were detected and isolated by HPLC, due to a stable chromatographic behavior of two peaks in response to the UV-B exposure. The different phenolic composition in those fractions could explain the differential antifungal activities of the extracts. In conclusion, UV-B exposure increases lemon peel natural defenses, by activating the differential synthesis of phenolic compounds and thereby raising the resistance against the phytopathogen.