INVESTIGADORES
RAPISARDA Viviana Andrea
congresos y reuniones científicas
Título:
POLYPHOSPHATE ROLE IN GLUCONACETOBACTER DIAZOTROPHICUS ABIOTIC STRESS RESISTANCE & BIOFILM FORMATION
Autor/es:
GRILLO-PUERTAS, M.; DELAPORTE-QUINTANA, P.; PEDRAZA, R. O.; RAPISARDA, V. A.
Lugar:
Córdoba
Reunión:
Congreso; LII Annual Meeting of SAIB (Argentine Society for Biochemistry and Molecular Biology); 2016
Institución organizadora:
SAIB
Resumen:
Gluconacetobacter diazotrophicus is a plant growth promoting bacterium able to colonize several plant species of economic importance. Its beneficial effects on plants are nitrogen-fixation, action against pathogens and mineral nutrient solubilization. The use of G. diazotrophicus as a biofertilizer requires a study of its physiological properties, including how this bacterium responds to the extracellular environment. We have previously shown that media phosphate (Pi) concentration modulate polyphosphate (polyP) levels in Escherichia coli cells affecting several cellular features. The aim of this work was to study the possible physiological changes in G. diazotrophicus Pal5 due to variations in growth media Pi concentration that may modulate intracellular polyP levels. Cells grown in high Pi reached higher OD values than those grown in sufficient Pi. In high Pi media, the cells accumulate high polyP levels and degraded it in stationary phase. This feature was accompanied with the improvement in survival, resistance to different stress agents (NaCl, H2O2, Cu(II)salts and copper-related fungicides) and biofilm formation in abiotic and biotic surfaces. Our findings on the relationship between media Pi concentration, resistance, biofilm formation and polyP levels contribute to elucidate the importance of Pi or its metabolites as signals for survival and colonization in this bacterium.