RAPISARDA Viviana Andrea
congresos y reuniones científicas
Postharvest oxidative treatment against the citric fruits pathogen Penicillium digitatum
CERIONI, L.; ROCHA, C. M. L.; PRADO, F. E.; WINIK, B. C.; RAPISARDA, V. A.; RODRÍGUEZ-MONTELONGO, L.
Mar del Plata
Congreso; XLIII Reunión Anual de SAIB; 2007
Infections by Penicillium digitatum and italicum generate the green and blue mold on citric fruits. In order to avoid the attack of these pathogens, fruits are intensely treated with synthetic fungicides, leading to the proliferation of resistant strains and to the international markets rejection of fresh fruits due to the increment of toxic residues. The aim of this work was to generate some alternative treatments to eliminate or to minimize the use of fungicides. Oxidative treatments with peroxides and heavy metal salts were applied on spores of P. digitatum. The results indicate that these spores lost viability when they were treated with CuSO4 and H2O2 after a brief incubation with NaClO. Thus, a lethal standard protocol (LSP) for the pathogen was elaborated, using 10 ppm NaClO, 6 mM CuSO4 and 50 mM H2O2. The spores treated with the LSP and analyzed by electron microscopy showed drastic alterations in cellular structures, although the damage was not observed at the wall level. In addition, a decrease in the infectivity of the spores was observed after this treatment. The oxidative procedure presented here, could be an important tool that may be applied in packinghouses, replacing the fungicides in the postharvest manipulation of fruits and protecting the human health and the environment.