RAPISARDA Viviana Andrea
congresos y reuniones científicas
Inorganic phosphate modulates biofilm formation and stress resistance in S. thyphimurium
OVEJERO, N. S.; GRILLO-PUERTAS, M.; RAPISARDA, V. A.; RINTOUL, M. R.
Congreso; XLVIII Annual Meeting of SAIB (Argentine Society for Biochemistry and Molecular Biology); 2012
In several organisms, inorganic polyphosphate (polyP) formation was shown to be critical for attributes such as motility, quorum sensing, biofilm formation, resistance to stress, and stationary phase survival.We have previously reported that, stationary cells grown in media containing at least 37 mM phosphate (Pi) maintain high polyP levels and high viability. They also exhibit resistance toH O and low capacity to form biofilm. Here, the effect of different Pi concentrations on cell viability, polyP level, oxidative stress resistance and biofilm formation capacity (BFC) was evaluated througout the growth curve of .Viability, oxidative stress resistance and polyP levels were high in stationary phase cells grown in high Pi concentration media (=25 mM). Biofilm formation in polystyrene, glass, polypropylene and stainless steel were evaluated in static condition at 30ºC with O?Toole and Kolter method. A preference for glass or polypropylene surfaces was observed. At 24 h, BFC was dependent on the Pi concentration of the planktonic preinocula and of the incubation media. BFC was maximal at 20mMPi. According to our results, we conclude that Pi concentration is an important signal for biofilm formation and stationary phase fitness in cells.