INVESTIGADORES
RABINOVICH Gabriel Adrian
congresos y reuniones científicas
Título:
Anti-galectin-1 antibodies are associated with the severity of ocular disease in autoimmune and infectious uveitis
Autor/es:
MARTA D. ROMERO, JUAN C. MUIÑO, GERMÁN A. BIANCO, MERCEDES FERRERO, CLAUDIO P. JUAREZ, JOSÉ D. LUNA, GABRIEL A. RABINOVICH
Lugar:
Córdoba, Argentina
Reunión:
Congreso; VII Latin American Congress of Immunology; 2005
Institución organizadora:
Latin American Society of Immunology
Resumen:
ANTI-GALECTIN-1 ANTIBODIES ARE ASSOCIATED WITH THE SEVERITY OF OCULAR DISEASE IN AUTOIMMUNE AND INFECTIOUS UVEITIS Marta D. Romero,  2 ,4 Juan C. Muiño, 3, 4 Germán A. Bianco, 1 Mercedes Ferrero, 2,4, Claudio P. Juarez, 4, José D. Luna 4, and Gabriel A. Rabinovich,  1 1 Immunogenetics, Faculty of Medicine, UBA,  2 Laboratory of  Immunopathology ,LIIDO,  3 Medicine III, Faculty of Medicine, UNC and 4 Fundación Ver. Córdoba, Argentina.   Background and objectives: Recent evidence indicates that galectin-1 (Gal-1)  plays critical roles in immunoregulatory processes. Administration of Gal-1 or its genetic delivery suppresses inflammation in experimental models of autoimmunity. Here we investigated the occurrence of anti-Gal-1 antibodies in patients with autoimmune and infectious uveitis. Methods: IgG, IgE and IgA anti-Gal-1 antibodies were assessed by ELISA and Western blot in sera from patients with autoimmune (n=47) and infectious (n=15) uveitis compared to healthy controls (n=30). Furthermore, anti-Gal-1 antibodies were eluted by incubating patient sera with nitrocellulose filters adsorbed with recombinant Gal-1. Results: IgE, IgG and IgA anti-Gal-1 antibodies were increased in sera from patients with autoimmune uveitis (p<0.001 vs controls), and in toxoplasmic retinochoroiditis (p< 0.001). The frequency of  IgE and IgG anti-Gal-1 antibodies was associated with progressive and poor outcome of disease. Furthermore,  eluted anti-Gal-1 antibodies immunoreacted with  retinal tissue in ELISA and in Western blot and  recognized photoreceptors in retinal sections.Conclusion: Anti-retinal Gal-1 antibodies are associated with the progression of ocular disease, suggesting their potential use for the follow-up of patients with uveitis.