UNTANGLING GALECTIN-MEDIATED CIRCUITS THAT CONTROL HYPOXIA-DRIVEN ANGIOGENESIS

NADIA BANNOUD; PABLO ALFREDO GARCIA; JULIAN GAMBARTE TUDELA; VICTORIA SUNDBLAD; ALEJANDRO CAGNONI; CAMILA BACH; JUAN MARTIN PEREZ SAEZ; ADA BLIDNER; SEBASTIAN MALLER; KARINA MARIÑO; MARIANA SALATINO; JUAN CERLIANI; GABRIEL RABINOVICH; DIEGO CROCI

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

SPRINGER NATURE

Año: 2022

1064-3745

Development of an aberrant vascular network is a hallmark of the multistep pathological process of tumor growth and metastasis. In response to hypoxia, several pro-angiogenic factors are synthesized to support vascularization programs required for cancer progression. Emerging data indicate the involvement of glycans and glycan-binding proteins as critical regulators of vascular circuits in health and disease. Galectins may be regulated by hypoxic conditions andcontrol angiogenesis in different physiopathological settings. These β-galactoside-binding proteinsmay promote sprouting angiogenesis by interacting with different glycosylated receptors and triggeringdistinct signaling pathways. Understanding the role of galectins in tumor neovascularization will contribute to the design of novel anti-angiogenic therapies aimed at complementing current anti-cancermodalities and overcoming resistance to these treatments. Here we describe selected strategies and methods used to study the role of hypoxia-regulated galectins in the regulation of blood vessel formation.