Cross-linked hyaluronan films loaded with acetazolamide–cyclodextrin–triethanolamine complexes for glaucoma treatment

CALLES, JAVIER ADRIÁN; MORA, MARIA JULIA; ONNAINTY, RENÉE; TARTARA, LUIS IGNACIO; GRANERO, GLADYS ESTER; LONGHI, MARCELA RAQUEL; DIEBOLD, YOLANDA; VALLÉS, ENRIQUE MARCELO; PALMA, SANTIAGO DANIEL

Therapeutic Delivery

FutureScience

Año: 2018 vol. 9 p. 205 - 220

2041-5990

Aim: This work aimed to design and characterize cross-linked hyaluronic acid?itaconic acid films loaded with acetazolamide?hydroxypropyl β cyclodextrin?triethanolamine complexes. Materials & methods: Films were cross-linked with itaconic acid and poly(ethyleneglycol)-diglycidylether. Biopharmaceutical properties were assessed by evaluating in vitro drug release rate, biocompatibility in a human corneal epithelial cell line, bioadhesiveness with pig gastric mucin, in vivo bioadhesion and efficacy. Results: Showed good mechanical properties and oxygen permeability. Proliferation rate of corneal cells was affected by highest acetazolamide concentration. Bioadhesive interaction exhibited a water movement from pig mucin to the film; in vivo experiments showed strong bioadhesion for 8 h and hypotensive effect for almost 20 h. Conclusion: Experimental set showed promising performance and encouraged future studies to optimize formulation.