Effects of ascorbate fatty ester derivatives on erythrocyte membrane lipoperoxidation.

M. I. SPENGLER; M. RASIA; S. PALMA; D. ALLEMANDI

CLINICAL HEMORHEOLOGY AND MICROCIRCULATION

IOS PRESS

Año: 2011 vol. 47 p. 163 - 168

1386-0291

6-O-alkyl ascorbic acid esters (ASCn) are amphiphilic molecules that behave as surfactants in aqueous solution. ASCn have shown some physical and rheological properties that suggest a potential utility as drug carriers. The present paper aims to evaluate the effect of ASCn on erythrocyte properties in order to get information regarding the relationship between osmotic fragility, erythrocyte deformability and membrane lipoperoxidation process. The assays were performed at the following concentrations: - the critical micelar concentration (CMC), -producing 10% hemolysis (CH10) and - producing 50% hemolysis (CH50). We observed that ASCn (ASC8, ASC10 and ASC12), at concentration nearby CMC, neither affected cell deformability nor produced lipoperoxidation. Nevertheless, at higher concentrations (CH10 and CH50), the RBCs incubated with ASCn were affected by a significant and progressive loss of deformability, simultaneously with an increase of osmotic fragility and membrane lipoperoxidation.10) and - producing 50% hemolysis (CH50). We observed that ASCn (ASC8, ASC10 and ASC12), at concentration nearby CMC, neither affected cell deformability nor produced lipoperoxidation. Nevertheless, at higher concentrations (CH10 and CH50), the RBCs incubated with ASCn were affected by a significant and progressive loss of deformability, simultaneously with an increase of osmotic fragility and membrane lipoperoxidation.10 and CH50), the RBCs incubated with ASCn were affected by a significant and progressive loss of deformability, simultaneously with an increase of osmotic fragility and membrane lipoperoxidation.