INVESTIGADORES
OUBIÑA Jose Raul
artículos
Título:
"GBV-C / HGV groups and subgroups: classification by an RFLP method based on the phylogenetic analysis of the 5' untranslated region".
Autor/es:
QUARLERI JF, MATHET VL, FELD M, FERRARIO D, DELLA LATTA MP, OUBIÑA JR; VERDUN R, SÁNCHEZ DO,
Revista:
JOURNAL OF CLINICAL MICROBIOLOGY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Lugar: Washington; Año: 1999 vol. 37 p. 1340 - 1347
ISSN:
0095-1137
Resumen:
.A phylogenetic tree based on 150 5* untranslated region sequences deposited in GenBank database allowed segregation of the sequences into three major groups, including two subgroups, i.e., 1, 2a, 2b, and 3, supported by bootstrap analysis. Restriction site analysis of these sequences predicted that HinfI and either AatII or AciI could be used for genomic typing with 99.4% accuracy. cDNA sequencing and subsequent alignment of 21 Argentine GB virus C/hepatitis G virus strains confirmed restriction fragment length polymorphism patterns theoretically predicted. This method may be useful for a rapid screening of samples when either epidemiological or transmission studies of this agent are carried out.
