INVESTIGADORES
OGGERO EBERHARDT Marcos Rafael
congresos y reuniones científicas
Título:
Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries
Autor/es:
CAMPERI, S. A.; IANUCCI, N. B.; ALBANESI, G.; OGGERO EBERHARDT, M.; ETCHEVERRIGARAY, M.; MESSEGUER, A.; ALBERICIO, F.; CASCONE, O.
Lugar:
Basilea. Suiza
Reunión:
Congreso; 11th European Congress on Biotechnology; 2003
Institución organizadora:
European Federation of Biotechnology
Resumen:
Peptide APAR was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti GM-CSF monoclonal antibody developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of µmol/ml, showed a maximum capacity of 9.1 mg MAb/ml and a dynamic capacity of 3.9 mg MAb/ml. 95 % yield of electrophoretically pure anti GM-CSF was obtained in a single step.
