INVESTIGADORES
OGGERO EBERHARDT Marcos Rafael
congresos y reuniones científicas
Título:
Characterization of a panel of GM CSF monoclonal antibodies
Autor/es:
OGGERO EBERHARDT, M.; KRATJE, R. B.; ETCHEVERRIGARAY, M.
Lugar:
La Habana. Cuba
Reunión:
Congreso; 8th International Scientific Congress on Medical Applications of Modern Biotechnology; 1999
Institución organizadora:
Centro de Ingeniería Genética y Biotecnología (CIGB)
Resumen:
Granulocyte-Macrofage Colony Stimulating Factor (GM-CSF) is a cytokine that regulates the proliferation and differentiation of hematopoietic progenitor cells, useful to reverse or prevent chemotheraphy secondary leukopenias. It was expressed in different host systems: bacteria, yeast and mammalian cells. All of them have biological activity. However, patients receiving non-glycosylated or partially glycosylated GM CSF develope antibodies that compromise the clinical efficacy of the trial (1). To study the undesirable immune response we produced a panel of E. coli derived recombinant human GM CSF (rhGM CSF) monoclonal antibodies (MAbs). We characterized the MAb´s immunochemical behaviour and neutralizing capacity in order to use this panel for evaluating the GM CSF epitopes involved in the therapeutic failure in humans.
