Cell Culture Evaluation Of Epo Neuroprotection And Neuroplasticity

BÜRGI-FISSOLO, M; APARICIO, G; KRATJE, R; SCORTICATI, C ; OGGERO, M

Copenhague

Congreso; 26th Meeting of the European Society for Animal Cell Technology (ESACT); 2019

European Society for Animal Cell Technology (ESACT)

Neurodegenerative diseases affect the nervous system with an incidence increasing every year. In this sense, human erythropoietin (EPO) has a leading role because its antiapoptotic, antiinflammatory, antioxidant and antiproliferative effects have been observed in neural tissues. Thus, the recombinant form of EPO (rEPO) might be a potential tool for neuroprotection and neurogenesis of brain-damaged areas. The in vitro neuroprotective activity of rEPO was determined by measuring the capacity of the cytokine to reverse the staurosporine (STP) induced apoptosis using a murine neuroblastoma cell line (N2a) and primary cultures from hippocampal neurons (HNPC). Neuroplasticity was determined by measuring neuritogenesis in N2a cells and filopodia and synapses formation in HNPC. The neuroprotection studies demonstrated that rEPO was capable to significantly reverse the STP-induced apoptosis in a dose-response way, both in N2a cells as in HNPC (between p