INVESTIGADORES
OGGERO EBERHARDT Marcos Rafael
artículos
Título:
Design and validation of an immuno-PCR assay for IFN-alpha2b quantification in human plasma
Autor/es:
ATTALLAH, CAROLINA; RODRIGUES, MA. CELESTE; LOZANO, VICTORIA; ETCHEVERRIGARAY MARINA; OGGERO, MARCOS
Revista:
BIOANALYSIS
Editorial:
Future Science
Referencias:
Lugar: Londres; Año: 2019
ISSN:
1757-6180
Resumen:
Aim. Nowadays, IFN-α is considered a promising therapeutic target for systemic lupus erythematosus. An immuno-PCR (iPCR) was developed to quantify low amounts of IFN-α in human plasma followed by a deeply analysis of the methodologic robustness throughout quality by design approach. Results. An accurate, sensitive, selective and versatile iPCR was validated. The critical iPCR procedural steps were identified, applying a Placket-Burman design. Also, this assay demonstrated an outstanding LOD of 0.3 pg/mL. A significate aspect relies on its high versatility to detect and quantify other cytokines in human plasma as the appropriate biotinylated antibody is employed. Conclusion. This reliable iPCR assay can be clinically used as an alternative method for quantitating and detecting low IFN-α2b concentrations in human plasma samples.
