INVESTIGADORES
OGGERO EBERHARDT Marcos Rafael
artículos
Título:
Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
Autor/es:
BOLLATI FOGOLÍN M. R.; OGGERO EBERHARDT, M.; KRATJE, R. B.; ETCHEVERRIGARAY, M.
Revista:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Editorial:
UNIV CATOLICA DE VALPARAISO
Referencias:
Lugar: Valparaíso; Año: 2002 vol. 5 p. 243 - 250
ISSN:
0717-3458
Resumen:
Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures.
