INVESTIGADORES
OGGERO EBERHARDT Marcos Rafael
artículos
Título:
Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries
Autor/es:
CAMPERI, S. A.; IANUCCI, N. B.; ALBANESI, G.; OGGERO EBERHARDT, M.; ETCHEVERRIGARAY, M.; MESSEGUER, A.; ALBERICIO, F.; CASCONE, O.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Dordrecht; Año: 2003 vol. 25 p. 1545 - 1548
ISSN:
0141-5492
Resumen:
The peptide, Ala-Pro-Ala_Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 µmol ml-1, showed a maximum capacity of 9.1 mg Mab ml-1 and a dynamic capacity of 3.9 mg Mab ml-1. A 95 % yield of electrophoretically pure anti-GM-CSF was obtained in a single step.
