INVESTIGADORES
OGGERO EBERHARDT Marcos Rafael
artículos
Título:
Rational selection of an antibody probe to detect the heterogeneous collection of CHO-derived rhGM CSF glycoforms
Autor/es:
OGGERO EBERHARDT, M.; FORNO, A. G.; KRATJE, R. B.; ETCHEVERRIGARAY, M.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Dordrecht; Año: 2006 vol. 28 p. 2049 - 2056
ISSN:
0141-5492
Resumen:
Six anti-E. coli rhGM-CSF monoclonal antibodies were generated using hybridoma technology. One of these showed identical affinity for the CHO and E. coli-derived cytokine (dissociation constants of 0.14 ± 0.01 nM and 0.13 ± 0.01 nM, respectively), mapping an epitope that is not hindered by carbohydrates. The antibody was used to develop a simple, specific and sensitive competitive ELISA to quantify the entire set of rhGM-CSF glycoforms (detection limit of 780 pg/ml) and it was successful as an affinity ligand to purify them. Therefore, this particular antibody is a useful, reliable and reagent for most immunochemical purposes with the aim of detecting, quantifying and purifying the highly heterogeneous collection of the CHO-derived rhGM-CSF isoforms.
