MIRANDA Leandro Andres
Expression of pejerrey gonadotropin-releasing hormone (pjGnRH): a study in higher teleostean fishes.
GUSTAVO SOMOZA,; DAVIS LESCHIED,; MIRANDA, LEANDRO ANDRES; LO NOSTRO, FABIANA; MAGLIULO CEPRIANO, LUCIA; ALEJANDRO MONTANER,; MARTIN SHREIBMAN,; JEAN RIVIER,; NANCY SHERWOOD,
BIOLOGY OF REPRODUCTION
SOC STUDY REPRODUCTION
Año: 2002 vol. 67 p. 18164 - 18164
Molecular variants of GnRH were characterized by reversephase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri]), and Atheriniformes (Patagonia pejerrey [Odontesthes hatchery]). Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish.