Brain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochemicSTROBL-MAZZULLA PH, MONCAUT NP, LÓPEZ GC, MIRANDA LA, CANARIO AVN, SOMOZA GM Brain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochem

STROBL MAZZULLA, PABLO; NATALIA MONCAUT,; GABRIELA LOPEZ,; MIRANDA, LEANDRO ANDRES; ADELINO CANARIO,; GUSTAVO SOMOZA,

GENERAL AND COMPARATIVE ENDOCRINOLOGY

Elsevier

Lugar: Orlando; Año: 2005 vol. 143 p. 21 - 32

0016-6480

The brain-type aromatase (P450aromB) cDNA from pejerrey Odontesthes bonariensis was characterized. Its sequence differs from the ovarian-derived aromatase (P450aromA) previously reported from the same species. The cDNA is 2305 bp in length and the deduced protein comprises 501 amino-acids. The percentage of identity was higher when compared to other brain-derived aromatase proteins (85 to 63%) and lower with ovarian-derived aromatases (64 to 57%). Both pejerrey aromatases shares just 60.8 % of identity. The tissue expression analysis showed that P450aromB was expressed in the kidney, brain, and pituitary gland of both sexes and also in the ovary, but not in the eye, spleen, liver, gill and testis. Semi-quantitative RT-PCR analysis of different male and female brain areas revealed that P450aromB was significantly higher expressed in the anterior male brain areas than in the corresponding female areas, and also when compared to posterior brain areas from both sexes. An immunological analysis using a polyclonal anti-teleost aromatase showed immunoreactive P450arom cells bordering the telencephalic ventricle and a strong signal in the ependymal cells of the preoptic area and the hypothalamus. In the optic tectum immunoreactive aromatase cells were labeled in the ventral wall and in the ependymal layer of the third and fourth ventricle with lateral projections. In the pituitary gland immunoreactive aromatase cells could be found in the rostral and proximal pars distalis. In this gland aromatase fibers were also detected in different areas of the pituitary gland; many of them concentrated around blood vessels.