MIRANDA Leandro Andres
Effects of estradiol and/or ethinylestradiol on sperm quality, fertilization, embryos and larvae survival of pejerrey fish Odontesthes bonariensis.
GARRIZ A; MENÉNDEZ-HELMANN RJ; MIRANDA LA
ELSEVIER SCIENCE BV
Lugar: Amsterdam; Año: 2015 vol. 167 p. 191 - 191
17 -Estradiol (E 2 ) and synthetic 17-Ethinylestradiol (EE 2 ) are estrogenic compounds present in surface waters as a consequence of municipal sewage discharges. The aim of this study was to evaluate the effects of E2, EE 2 and its mixtures on different reproductive parameters and embryo?larval survival in pejerreyﬁsh ( Odontesthes bonariensis). In order to analyze the effect of these compounds on sperm quality, fertil-ization%, embryo?larval survival (%), and the point of no return (PNR), different assays were performed using concentrations 175, 350, 700 and 1400 ng/L of E 2 ; 22.5, 45, 90 and 180 ng/L of EE 2 and mixtures M1(175 E2 + 22.5 EE 2, ng/L), M2 (350 E 2 + 45 EE 2, ng/L), M3 (700 E2 + 90 EE 2, ng/L) and M4 (1400 E2 + 180 EE2 ng/L). No signiﬁcant differences in motility parameters were observed between E2 and EE 2 treatments and the control group. However, a signiﬁcant decrease in motility% was recorded for all mixtures tested compared with the control samples. For fertilization%, only sperm activated with M4 showed a signiﬁcant decrease compared with the control group. In the case of embryo survival, there was only a signiﬁcant decrease in the highest concentration of EE2 compared with the control group. For the mixtures, M3 isthe one that had the most adverse effect on embryo survival. In larval survival, there was a signiﬁcant decrease in concentration 175 and 700 ng/L of E 2 compared with the control group. In EE 2 treatments, the ones with a signiﬁcant reduction in larval survival were concentration 45 and 90 ng/L. And for the mixture treatments, M1, M3 and M4 had a signiﬁcantly lower larval survival than the control group. In comparison to other treatments, M1 demonstrated a signiﬁcant difference in PNR when compared with the control group. The results obtained demonstrated that the exposure to mixtures of E2 and EE2 affected ﬁsh sperm motility, fertilization% and, embryo and larval survival even at relevant environmental concentrations highlighting the necessity of considering the effects of pollutants mixtures in ecotoxicological studies.