MIRANDA Leandro Andres
Subcellular Energy Balance of Odontesthes bonariensis Exposed to a Glyphosate-based Herbicide. Ecotoxicology and Environmental Safety
MENÉNDEZ-HELMANN RJ; MIRANDA LA; SALIBIÁN A
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ACADEMIC PRESS INC ELSEVIER SCIENCE
Lugar: Amsterdam; Año: 2015 vol. 114 p. 157 - 157
Water pollution by agrochemicals is currently one of the most critical problems for the conservation of aquatic ecosystems. Glyphosate [N-(phosphonomethyl) glycine); PMG] is the main broad-spectrum post emergence herbicide used for the control of a wide range of pests in soybean crops. Adenylate energy charge (AEC) reﬂects the energy balance of the cells, a measure of the energy available from the ade-nylate pool: adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Background adenylates, phosphagens and the AEC index of two year old Odontesthes bonariensis were determined in some tissues by HPLC, and the impact on subcellular energy balance of sublethal glyphosate-based herbicide exposure was analyzed. The doses used were 0 (control tank), 1 or 10 mg PMG L 1, trials were carried out during 15 days. AEC values in brain, liver and muscle from control ﬁsh were 0.37 7 0.02, 0.4 9 7 0.05 and 0.56 7 0.03, respectively (means 7 SEM). While brain ATP concentra-tions were undetectable (hence low values of AEC), the muscle tissue showed the highest concentrationsof the more energetic molecules: 0.18 μ mole ATP g 1 and 8 μ mole phosphocreatine g 1(PCr g 1). In the brain, no signiﬁcant changes were detected in exposed ﬁsh compared to controls. Instead, in both the liver and muscle of animals exposed to the highest concentration of the herbicide, signi ﬁ cant changes in the AEC (reduction of 26% and 15%, p o 0.05) with respect to the control group were determined. Chronic exposure (15 days) of Odontesthes bonariensis to 1 and 10 mg L 1 of formulated glyphosate did not affect brain AEC. However, the highest concentration of the herbicide produced a signi ﬁcant decrease in liver and muscle AEC manifesting adverse sublethal effects on the energy metabolism. These results suggestthe usefulness of AEC as a biomarker of ﬁsh glyphosate exposure.