INVESTIGADORES
MATHET Veronica Lidia
artículos
Título:
In vitro detection of dissimilar amounts of Hepatitis C virus (HCV) subtype -specific RNA genomes in mixes prepared from sera of persons infected with a single HCV genotype
Autor/es:
QUARLERI JF, ; BUSSY MV, ; MATHET VL, ; RUIZ V, ; IÁCONO R, ; LU L, ; ROBERTSON BH, ; OUBIÑA JR
Revista:
JOURNAL OF CLINICAL MICROBIOLOGY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Lugar: Washington; Año: 2003 vol. 41 p. 2727 - 2733
ISSN:
0095-1137
Resumen:
The level of in vitro detection of viral genomes in mixes with two different hepatitis C virus (HCV) subtypes
was investigated by artificially mixing previously measured subtype-specific HCV RNA genomes. The RNAs in
these mixtures were reverse transcribed and then PCR amplified by using two sets of primers corresponding
to the 5 untranslated region and digested with endonucleases to analyze the restriction fragment length
polymorphism patterns. This approach facilitated detection of a wider range of type-specific HCV genomes
than originally described, beyond equimolar concentrations of contributing HCV subtypes. Moreover, by using
computerized image analysis, this study also demonstrated that the true contribution of each virus typeand
consequently of mixed infectionsmay be underestimated when only visual observation is carried out. These
results may be useful for comparing data obtained from this and other currently used methodologies. untranslated region and digested with endonucleases to analyze the restriction fragment length
polymorphism patterns. This approach facilitated detection of a wider range of type-specific HCV genomes
than originally described, beyond equimolar concentrations of contributing HCV subtypes. Moreover, by using
computerized image analysis, this study also demonstrated that the true contribution of each virus typeand
consequently of mixed infectionsmay be underestimated when only visual observation is carried out. These
results may be useful for comparing data obtained from this and other currently used methodologies.