New phylogenetic insights into Saprolegniales (Oomycota, Straminipila) based upon studies of specimens isolated from Brazil and Argentina 1 & 2 & Agostina V. Marano1 & José I. de Souza1

SARAH C. O. ROCHA; CLAUDIA C. LOPEZ-LASTRA; AGOSTINA MARANO; IVANILDO DE SOUZA; MANUEL E. RUEDA PÁRAMO ,; CARMEN PIRES ZOTARELLI

MYCOLOGICAL PROGRESS

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2018

1617-416X

Saprolegniales is a complex and monophyletic order of oomycetes. Their members inhabit terrestrial, freshwater, and marineecosystems and have a worldwide distribution. In these ecosystems, they are found as saprobes, parasites, or even pathogens ofanimals and plants of economic importance. In this study, a concatenate phylogeny of the partial LSU and complete ITS rDNAregions is presented, including isolates from Brazil and Argentina, which were sequenced after a detailed morphological analysis.Among the sequenced species, Achlya orion, Leptolegnia eccentrica, Phragmosporangium uniseriatum, and Pythiopsisirregularis are included for the first time in a phylogeny. Our results are in agreement with the recent informal proposals outlinedin taxonomic overviews of the Oomycota of G.W. Beakes and collaborators, who placed the family Verrucalvaceae into theSaprolegniales and introduced the family Achlyaceae to group Achlya s.s., Brevilegnia, Dictyuchus, and Thraustotheca. Theseresults also support the transference of Achlya androgyna to Newbya. Leptolegnia appears as paraphyletic, with the separation ofL. eccentrica from the other species of this genus. In addition, Phragmosporangium, which is herein sequenced for the first time,clustered as sister to some species of Aphanomyces, including the type species, A. stellatus.Keywords Achlyaceae . Morphology . Phylogeny . Saprolegniaceae . VerrucalvaceaeIntroductionSaprolegniales is a monophyletic order within the phylumOomycota (Straminipila), which groups biflagellate heterotrophicorganisms that have eucarpic, mycelial and coenocyticthalli of