Effect of isolated compounds from sugar cane leaves on seedling growth of L. sativa.

SAMPIETRO D., VATTUONE M., ISLA M.I

Tucumán

Congreso; Bio Cell 29(1) 109 (2005)-XXI Jornadas Científicas de la Asociación de Biología de Tucumán; 2004

Asociación de Biología de Tucumán

Allelopathy is the direct action (negative or positive) of a compound released from a plant on the growth and/or development of other plant. Recently in a bioassay-guided isolated we isolated vanillic (VA), syringic (SA) and ferulic acids (FA). The aim of this work was to evaluate the phytotoxic effect of these acids alone and in mixtures. Seeds of L.sativa were placed on plates of 24 wells, on Whatman N 1 paper discs. The phenolic acids alone, in binary mixtures and in a ternary mixture were assayed between 0.1 and 2.5 mM. Plates were placed on a growth chamber (25±1 °C with a 12 h photoperiod at 400 µgmol/m2.s, PAR). FA, SA and VA inhibited the radicle elongation at concentrations beyond 0.1 mM. The concentrations needed to reduced 50% the radicle elongation (IC50) were 0.75±0.04 mM (VA), 2.25±0.08 mM (FA) and 2.50±0.07 mM (SA). The IC50 of the mixtures were 0,90±0.12 mM (SA+VA), 1.20±0.10 mM (FA+VA), 2.50±0.20 mM (SA+FA) and 1.40±0.17 mM (FA+VA+SA). VA was the most inhibitory on radicle elongation of L. Sativa. The phenolic acid were less phytotoxic assayed in mixtures then alone, suggesting an antagonistic interaction among them. More studies about the impact of this compounds on the agroecosystem are in progress.