INVESTIGADORES
IGLESIAS Alberto Alvaro
congresos y reuniones científicas
Título:
Monofluorophosphate Inhibits the Key Step of Internal Polysaccharide Biosynthesis in Streptococcus mutans
Autor/es:
M.D. ASENCIÓN DIEZ; A.M. DEMONTE; C. NALEWAY; A.A. IGLESIAS; M.A. BALLICORA
Lugar:
Boston
Reunión:
Congreso; Annual Meeting ASBMB; 2013
Institución organizadora:
American Society for Biochemistry and Molecular Biology
Resumen:
Streptococcus mutans is the leading cause of dental caries worldwide and accumulates a glycogen-like internal polysaccharide (IPS) which contributes to cariogenicity when grows in excess of sugars. Previously, we characterized the ADP-glucose pyrophosphorylase from S. mutans belonging to the key step leading to IPS synthesis. The enzyme showed two active conformations, homotetrameric GlgC and heterotetrameric GlgC/GlgD,the latter being one magnitude order more active and having different regulatoryproperties. Also, sodium monofluorophosphate (MFP) is the toothpastes active compound with established anticariogenic properties, although the molecular target and/or its mechanism are still unknown. In this work, we show that MFP partially inhibits both ADPGlc PPase active conformations.