INVESTIGADORES
IGLESIAS Alberto Alvaro
artículos
Título:
The Kinetic Properties of Liver Glucokinase and Its Function in Glucose Physiology as a Model for the Comprehensive Study of Enzyme’s Kinetic Parameters and Reversible Inhibitors
Autor/es:
D.M. BUSTOS Y A.A. IGLESIAS
Revista:
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
Editorial:
Elsevier Science
Referencias:
Año: 2000 vol. 28 p. 332 - 337
ISSN:
1470-8175
Resumen:
AbstractGlucokinase is a key enzyme in carbohydrate metabolism in mammals. Particularly relevant is the involvement of the liver enzyme for the maintenance of blood glucose concentrations. In playing this key role, the occurrence of normal kinetic parameters of liver glucokinase is critical. Modi"cation of K. and/or <.!9 results in pathological situations occurring with hypo- or hyperglycemia. Based on this, an exercise is proposed in which di!erent mice cell lines with abnormalities in glucose utilization are analyzed to determine the metabolic alteration at a molecular level. The student must determine the cause of abnormality by processing thekinetic data. Also, the characterization of di!erent compounds that are reversible inhibitors of the enzyme from kinetic data is required to identify a drug that can correct the pathology in each case. One major objective of the problem is to learn about the meaning of kinetic parameters of enzymes, as well as the action of di!erent types of reversible inhibitors. ( 2001 IUBMB. Published by Elsevier Science Ltd. All rights reserved.