INVESTIGADORES
GORLA Nora Bibiana Maria
artículos
Título:
Hypomethylation in the C-MYC oncogene in colon carcinoma
Autor/es:
LARRIPA IRENE; GORLA NORA; GUTIERREZ MARINA; BEMEDO. ; A. JMELNITZKY; CHOPITA N; SLAVUTSKY IRMA
Revista:
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH : CR.
Editorial:
Springer
Referencias:
Año: 1993
ISSN:
0392-9078
Resumen:
The methylation State of the third exon of c-myc oncogene was studied in ten human colon carcinomas and adjacent normal tissue. DNA methylation was studied by means of two restriction endonuclease isoschizomers Msp I and Hpa II which recognize the sequence 5?- CCGG-3?. In 80% of the cases digested with Hpa Hit was observed the presence of two bands of 2.2 Kb and 3.4 Kb. Taking into account the behaviour of this enzime an hypomethylation in the CCGG site in the third exon was present. In normal DNA a higher molecular weigh ~ md (5.6 Kb) was observed indicatl ing a complete methylation in this site. i´he inverse correlation between gene expression and DNA SS methylation can be related with the oncogene activation in tumoral development.
