INVESTIGADORES
GONZALEZ LEBRERO Rodolfo Martin
congresos y reuniones científicas
Título:
Nucleotide specificity in the presence of RNA by dengue virus NS3 helicase
Autor/es:
LA CABABIE; JJ INCICCO; LG GEBHARD; AV GAMARNIK; RM. GONZÁLEZ LEBRERO; SB. KAUFMAN
Lugar:
Carlos Paz
Reunión:
Congreso; XLII reunión anual de la Sociedad Argentina de Biofísica; 2013
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
The dengue virus genome encodes a polyprotein which is cleaved by peptidases to give three structural and seven non-structural (NS) proteins. Among these the NS3 protein presents the characteristic features of helicases.This protein moves along single strands and unwinds double strands of RNA molecules driven by the binding and hydrolysis of nucleosides triphosphates (NTPs). It is known that this enzyme catalyzes the hydrolysis of several NTPs and this activity is enhanced by the presence of single stranded RNA. We have previously studied the specificity of NS3 for nucleotides ATP, GTP, CTP and UTP in the absence of RNA and now we examine how this specificity is affected by the presence of RNA. For this purpose we obtained the substrate curves for the four nucleotides mentioned in the presence of poly(A). In all cases it was observed lower KM and higher kcat values than in absence of RNA. According to the value of the specificity parameter (kcat / KM) we obtained the following specificity order: ATP> GTP> CTP> UTP.With grants from: Universidad de Buenos Aires UBACyT, CONICET and ANPCyT.