INVESTIGADORES
GEFFNER Jorge Raul
artículos
Título:
Respiratory syncytial virus (RSV) infects CD4+ T cells: Frequency of circulating CD4+ RSV+ T cells as a marker of disease severity in young children
Autor/es:
RAIDEN, SILVINA; SANANEZ, INÉS; REMES-LENICOV, FEDERICO; PANDOLFI, JULIETA; ROMERO, CECILIA; DE LILLO, LEONARDO; CEBALLOS, ANA; GEFFNER, JORGE; ARRUVITO, LOURDES
Revista:
JOURNAL OF INFECTIOUS DISEASES
Editorial:
UNIV CHICAGO PRESS
Referencias:
Año: 2017 vol. 215 p. 1049 - 1058
ISSN:
0022-1899
Resumen:
Background. Although human airway epithelial cells are the main target of respiratory syncytial virus (RSV), it also infects immune cells, such as macrophages and B cells. Whether T cells are permissive to RSV infection is unknown. We sought to analyze the permissiveness of CD4+ T cells to RSV infection. Methods. CD4+ and CD8+ T cells from cord blood, healthy young children, and adults were challenged by RSV or cocultured with infected HEp-2 cells. Infection, phenotype, and cytokine production by T cells were analyzed by flow cytometry or enzymelinked immunosorbent assay. Expression of RSV antigens by circulating CD4+ T cells from infected children was analyzed by flow cytometry, and disease severity was defined by standard criteria. Results. CD4+ and CD8+ T cells were productively infected by RSV. Infection decreased interleukin 2 and interferon ,production as well as the expression of CD25 and Ki-67 by activated CD4+ T cells. Respiratory syncytial virus antigens were detected in circulating CD4+ and CD8+ T cells during severe RSV infection of young children. Interestingly, the frequency of CD4+ RSV+ T cells positively correlated with disease severity. Conclusions. Respiratory syncytial virus infects CD4+ and CD8+ T cells and compromises T-cell function. The frequency of circulating CD4+ RSV+ T cells might represent a novel marker of severe infection.