INVESTIGADORES
GEFFNER Jorge Raul
artículos
Título:
In vitro susceptibility of CD4+ and CD8+ T cell subsets to fludarabine.
Autor/es:
GAMBERALE R, GALMARINI CM, FERNÁNDEZ-CALOTTI P, JORDHEIM L, SÁNCHEZ-AVALOS J, DUMONTET C, GEFFNER J, GIORDANO M.
Revista:
BIOCHEMICAL PHARMACOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2003 vol. 66 p. 2185 - 2191
ISSN:
0006-2952
Resumen:
Administration of the adenosine analogue fludarabine (FLU) in vivo induces a profound and prolonged T lymphopenia which mainly affects CD4(+) cells. To better understand the mechanistic basis underlying this preferential depletion, we analyzed the in vitro susceptibility of T cell subsets to FLU-induced apoptosis. Contrasting with observations in vivo, our results showed that treatment of peripheral blood mononuclear cells with FLU induced a higher level of apoptosis in CD8(+) than in CD4(+) T lymphocytes. This increased sensitivity of CD8(+) T cells to FLU was observed in samples from both, healthy donors and B cell chronic lymphocytic leukemia patients, and resulted in higher CD4:CD8 ratios in FLU-treated than in untreated cultures (P