BETA-CYCLODENTRIN COATED MAGNETIC NANOPARTICLES: NEW INSIGHTS FOR THE LOCALIZED TREATMENT OF DIVERSE PATHOLOGIES.

MARIELA AGOTEGARAY; VIRGINIA MASSHEIMER; ELBA GARCÍA; VERÓNICA LASSALLE; ADRIÁN CAMPELO

Mar del Plata

Encuentro; VII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE NANOMEDICINA (NANOMEDAR); 2016

Magnetic nanoparticles(MNPs) provide new insights for targeted drug delivery to a specific site in the organism by an external magnetic field. Coating of MNPs improves biocompatibility and provides a platform to attach drugs for diverse purposes. ObjectiveSynthesis, characterization and evaluation of endothelial cytotoxicity of nano-systems composed of magnetite(MG), oleic acid(OA) and betacyclodextrin(BCD) as nano-devices for drug targeting.Design OA stabilized MG MNPs were synthesized by co-precipitation. A dispersion of MAG-OA (0.5 mg/mL) in hexane was mixed with aqueous solution of BCD in different MNPs:BCD ratios (1:1; 1:2; 1:3, named MG-OA-BCD1, MG-OA-BCD2, MG-OA-BCD3) for 24h at room temperature. The organic phase was separated; the content of the aqueous phase was extracted with Nd magnet, washed and dried. Samples were studied by FTIR, DLS to determine hydrodynamic diameter(Dh) and surface charge(z); TEM and HR-TEM. Primary cultures of Rat aortic endothelial cells(EC) were exposed 48h to final concentrations of 1, 10 and 100 µg/mL of MG-OA-BCD1 and MG-OA-BCD2. Cell viability was evaluated by MTT assay and by the capacity to produce NO(DAN assay).ResultsFTIR demonstrated the incorporation of BCD on MG-OA. Aqueous monodisperse MG-OA-BCD1, MG-OA-BCD2, MG-OA-BCD3 presented Dh of 589.3±42.71nm, 370.3±6.00nm, 555.4±8.50nm and z of 2.27±6.14mV, 10.3±4.85mV, 14.4±5.12mV respectively. HR-TEM micrographs showed almost-spherical shaped MNPs. Synthesis of MG-OA-BCD3 was not reproducible. Cell viability was not affected at doses of 10µg/mL for MG-OA-BCD1 and 1 µg/mL for MG-OA-BCD2(p