INQUISUR   21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
OPTIMIZATION OF A HPLC METHOD FOR THE SIMULTANEOUS DETERMINACION OF AMOXICILLIN AND SULBACTAM PIVOXIL IN PHARMACEUTICAL FORMULATIONS
Autor/es:
F. CABRERA; M. F. RAZUC; M. V. RAMIREZ RIGO
Lugar:
Rosario
Reunión:
Congreso; 4ta. Reunión Internacional de Ciencias Farmacéuticas; 2016
Resumen:
Introduction:The combination of Amoxicillin-AMOX and Sulbactam pivoxil-SULB is administrated as tablets and oral suspensions in the treatment of bacterial infections. The quality control of these formulations included, among others, uniformity of content and dissolution test. There is not pharmacopoeial methods to determine Sulbactam pivoxil alone or combined. Although Qi et al.(2003) proposed the simultaneous determination of both drugs using HPLC and UV-determination, the area ratio is very high (>8) what make it difficult to use it mainly in the dissolution test. The purpose of this work is to optimize the operative conditions of a HPLC method for the simultaneous determination of AMOX and SULB, in term of area ratio and retention time.Materials and methods:A HPLC Perkin Elmer-200 with UV detector and a C18 column (150x4.6mm, 5µm) were used. A acetonitrile:water mobile phase was delivered at a flow rate of 1.0 mL.min-1. A 22design with central point was selected for subsequent optimization. Acetonitrile content in the mobile phase (20-80%) and wavelength of detection (205-230nm) weremodified. The retention time, area ratio and heights ratio were analyzed. An interference study of excipients was carried out.Results:The effect of the studied factors on all the analyzed response were significant (pvalues