. ?Folic acid conjugated magnetic nanoparticles as theranostics: An insight on their behavior in simulated physiological media?

PAMELA AZCONA; VERÓNICA LASSALLE

Workshop; Summer School & International Workshop on Advanced Materials Challenges for Health and Alternative Energy Solutions; 2016

Folic acid conjugated magnetic nanoparticles as theranostics: An insight on their behavior in simulated physiological media.Pamela Azcona and Verónica LassalleINQUISUR-UNS-CONICET, Avda. Alem 1253 (8000) Bahía Blanca, Argentina.Magnetic nanoparticles (NPMs) composed of magnetite (MAG) stabilized with sodiumdodecylsulfate were functionalizated with amino propyl trietoxysilane (APTS) aiming to achieve aminated ?MNPs. The goal was to incorporate folic acid (FA) molecules in order to provide specificity to earlier detection of tumoral cells and tissues. The covalent FA linkage was attained using N,N'-Dicyclohexylcarbodiimideas coupling agent. Physicochemical properties were evaluated by TGA, FTIR, TEM, hydrodynamic diameter (Dh) and Z potential measurements. NPMs-FA were tested regarding to their stability, analyzing two criterias: i-The alteration in the hydrodynamic size as a function of the storage time and ii-the leaching of FA as a function of the time when nanoparticles were incubated in a media simulating the biological one. It was found that the formulations maintained their HD along 15 days, retaining FA on their surfaces. The feasibility of these formulations to interact with biological fluids was also assayed. To this end incubation of MNPs-FA with a media simulating the biological one was implemented. Bovine serum albumin was used as model of predominant protein in physiological fluid that was composed of Ringer Solution (RS). The characterization of the MNPs-FA after treatment revealed that BSA was successfully bonded to the NPs surface.This fact conferred extra stability to the NPs in terms of the aggregates formation in the physiological med