Subacute tissue biodistribution of magnetic nanoparticles functionalized with chitosan intended for drug delivery systems

MARIELA A. AGOTEGARAY; PAMELA L. AZCONA; ROBERTO D. ZYSLER; ADRIAN CAMPELO; VIRGINIA L. MASSHEIMER; FERNANDA A. GUMILAR; ALEJANDRA MINETTI; VERÓNICA L. LASSALLE

Rosario

Congreso; Congreso Latinoamericano de Órganos Artificiales, Biomateriales e Ingeniería de Tejidos; 2014

Sociedad Latinoamericana de Biomateriales, Organos Artificiales e Ingenieria de Tejidos (SLABO)

Nanoparticles are described as materials with three external equal nanoscale dimensions. Magnetic nanoparticles (MNPs) present the special characteristic associated to the feasibility of external manipulation under the influence of an external magnetic field. An interesting and promising application lies in the use of MNPs as drug targeted delivery systems by directing the drug to the desired part of the body. This would avoid side effects associated to the free form of the drug, increasing circulation times and reducing toxicity. Two magnetic nanodevices intended for targeted drug delivery systems were evaluated in mice to study their tissue distribution after a subacute exposure. Formulations consist in a magnetic core composed by magnetite functionalized with oleic acid and recovered with crosslinked and non-crosslinked chitosan (N1 and N2, respectively). N1 and N2 were intraperitoneal administered once a week for four weeks. A control group was administered with saline solution (n = 8 per group). Doses employed were about 33 mg MNPs/kg. Then animals were sacrificed by cervical dislocation and the organs such as liver, spleen, kidneys, lungs, brain and heart were analyzed by VSM (Vibrating Sample Magnetometry) to determine the content of the MNPs by magnetism measurements avoiding the physiological contribution of endogenous iron. The care and the handling of the animals were in accordance with the internationally accepted standard Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Institute of Health and CICUAE (Comité Institucional para el Cuidado y Uso de Animales de Experimentación ? Universidad Nacional del Sur).